Thyroglobulin quantitation by mass spectrometry

Abstract

Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve adding an internal standard to the test sample; digesting thyroglobulin from the test sample and the internal standard to form thyroglobulin peptides; purifying the thyroglobulin peptides; ionizing the thyroglobulin peptides to generate one or more ions detectable by mass spectrometry; quantifying the amount of the one or more ions by mass spectrometry, wherein the one or more ions comprise an ion having a mass/charge ratio of 541.3±0.5, 612.3±0.5, 636.4±0.5, 726.4±0.5, 797.4±0.5, 912.4±0.5, and/or 1059.5±0.5; and relating the amount of the one or more ions to the amount of thyroglobulin in the test sample.

Dependencies are inferred from the claim text.