Method and system for buoyant-particle-assisted cell therapy

A method for buoyancy-assisted production of cells for a cell therapy, the method comprising:

• performing a first negative selection, comprising:
• producing bound T cells by combining a first set of buoyant particles and T cells within a container, wherein the T cells comprise a first subset of the T cells and a second subset of the T cells, wherein the first set of buoyant particles are functionalized with moieties for binding to and forming a complex with the second subset of the T cells, wherein the bound T cells aggregate at a superior region of the container;
• and
• removing the bound T cells;
• culturing the first subset of the T cells in the container to produce expanded T cells;
• performing a second negative selection, comprising:
• producing second bound T cells by adding a second set of buoyant particles to the container, wherein the expanded T cells comprises a first subset of the expanded T cells and a second subset of the expanded T cells, wherein the second set of buoyant particles are functionalized with moieties for binding to and forming a complex with the second subset of the expanded T cells, wherein the second bound T cells aggregate at a superior region of the container;
• and
• removing the second bound T cells;
• and
• collecting the first subset of the expanded T cells for the cell therapy.