Antigens Derived from Citrullinated 14-3-3 and Uses Thereof in the Diagnosis of Rheumatoid Arthritis
AssigneeAugurex Life Sciences Corp.
InventorAnthony Marotta
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Abstract
The present invention provides citrullinated 14-3-3η peptides and antibodies thereto and methods of using same to evaluate arthritic conditions such as rheumatoid arthritis.
Claims20
An isolated citrullinated 14-3-3 eta protein or fragment thereof specifically recognized by anti-14-3-3 eta autoantibodies present in the serum of a patient suffering from an arthritic condition.
A method for diagnosing an arthritic condition in a subject comprising contacting a biological sample from the subject with at least one citrullinated 14-3-3 protein or fragment thereof under a condition suitable for the formation of at least one immune complex between the citrullinated 14-3-3 protein or fragment thereof and autoantibodies against the citrullinated 14-3-3 protein or fragment thereof that may be present in the biological sample;
- and detecting the presence of immune complexes between the citrullinated 14-3-3 protein or fragment thereof and autoantibodies against the citrullinated 14-3-3 protein or fragment thereof;
- wherein said presence of said immune complexes is indicative of an arthritic condition in said subject.
A method for diagnosing and/or prognosing an arthritic condition in a subject comprising
- contacting a biological sample from the subject with at least one antibody capable of binding selectively to a citrullinated human 14-3-3 protein or a fragment thereof under a condition suitable for the formation of at least one immune complex between the antibody and citrullinated 14-3-3 proteins that may be present in the biological sample;
- and
- detecting the presence, degree and/or location of at least one citrulline residue within said citrullinated 14-3-3 protein,
- wherein said presence, degree and/or location of said at least one citrulline residue is indicative of an arthritic condition in said subject or informative of a prognosis for said subject.
Dependencies are inferred from the claim text.