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US 2025/0093342 A1Published application
Analyzing cell samples
AssigneeSingular Genomics Systems, Inc.
InventorEli N. Glezer
Life of this patent
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Abstract
Disclosed herein, inter alia, are methods and compositions useful for detecting multiple biological targets.
Claims20
A method of analyzing a sample, the method comprising:
- depositing a sample comprising a cell on a solid support;
- contacting the sample with a first probe and a second probe, thereby forming a first complex and a second complex, wherein
- the first complex comprises the first probe bound to a first organelle, wherein said first probe comprises a first fluorescent dye attached to the first probe via a cleavable linker;
- the second complex comprises the second probe bound to a second organelle, wherein said second probe comprises a second fluorescent dye attached to the second probe via a covalent linker;
- directing an excitation light to sample and detecting an emission light from the first fluorescent dye;
- and
- cleaving the cleavable linker and the directing an excitation light to sample and detecting an emission light from the second fluorescent dye.
4 more elements
A device configured to analyze a sample, said device comprising
- a solid support comprising the sample, wherein the sample comprises a cell;
- a first probe bound to a first organelle in the cell, wherein said first probe comprises a first fluorescent dye attached to the first probe via a cleavable linker;
- a second probe bound to a second organelle in the cell, wherein said second probe comprises a second fluorescent dye attached to the second probe via a covalent linker;
- a light source configured to provide excitation light having a wavelength between 200 nm to 1500 nm;
- and
- an image sensor configured to detect fluorescent emissions from the sample;
- wherein the solid support forms part of a flow cell.
4 more elements
Dependencies are inferred from the claim text.