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US 2025/0093339 A1Published application
Compositions and methods for enhanced sample multiplexing
AssigneeBiolegend, Inc.
InventorsAndre VIEIRA ZORZETTO FERNANDES, Ze SHI, Zeinab MOUSSA, Fernando GRIGERA
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Abstract
Provided herein are compositions, methods and systems that allow for detection of samples via compositions that bind ubiquitous ligands or targets, to improve the capability of multi-sample utilization in single cell sequencing.
Claims21
1-38. (canceled)
A method comprising:
- (a) contacting a plurality of target cells comprising one or more glycans with a first plurality of complexes, wherein:
- the first plurality of complexes comprise a first glycan-binding agent and a first labeling oligonucleotide,
- the first glycan-binding agent binds to all or a portion of a first glycan on the one or more target cells, and
- the first glycan-binding agent is a first lectin;
- (b) detecting the first labeling oligonucleotide;
- and
- (c) identifying a first glycan on the one or more target cells according to the detection of the first labeling oligonucleotide.
4 more elements
Dependencies are inferred from the claim text.