Method for estimating abundance and distribution features of antibiotic resistance genes in surficial sediments of lake and reservoir

A method for estimating abundance and distribution features of antibiotic resistance genes (ARGs) in surfacial sediments of lake and reservoir, comprising:

• a) obtaining an annual input total of nitrogen and phosphorus pollutants of each tributary flowing into the lake and reservoir;
• b) obtaining abundance of each type of ARGs in the 0˜10 cm sediment of a surface layer of the lake and reservoir in the next year, and analyzing correlation between the abundance of ARGs and the annual input total of the surrounding nitrogen and phosphorus pollutants by using a geographical weighted regression model, so as to construct a linear regression equation between the abundance of each type of ARGs and the nitrogen and phosphorus discharge;
• and
• c) calculating annual input total of nitrogen and phosphorus pollutants to be estimated for each geographical location of the lake and reservoir using inverse distance weighting interpolation analysis based on the annual input total of the nitrogen and phosphorus pollutants obtained in step a, and substituting the calculated annual input total of the nitrogen and phosphorus pollutants to be estimated into the linear regression equation constructed in step b to estimate the abundance of each type of ARGs corresponding to the geographical location of the lake and reservoir and analyze a distribution feature of the ARGs of the lake and reservoir.