Patterned Flow Cell and Preparation Method Thereof, and Related Sequencing Method
AssigneesSequlite Genomics US, Inc., Guangdong Runpeng Biological Technology Co., LTD.
InventorXiongwei YAN
Life of this patent
Estimating the term…
Abstract
Provided are a patterned flow cell and a preparation method thereof, and a related sequencing method. The method for preparing a patterned flow cell includes: a) providing a flow cell substrate, wherein the flow cell substrate contains binding regions, and the binding regions are arranged in a patterned manner on the flow cell substrate; b) reacting a reaction reagent with the binding regions to obtain a substrate bound with a linker, wherein the linker contains a halogen atom from the reaction reagent; and c) connecting the substrate bound with the linker with a first nucleic acid to obtain a patterned flow cell.
Claims20
A method for preparing a patterned flow cell, comprising:
- a) providing a flow cell substrate, wherein the flow cell substrate comprises binding regions, and the binding regions are arranged in a patterned manner on the flow cell substrate;
- b) reacting a reaction reagent with the binding regions to obtain a substrate bound with a linker, wherein the linker comprises a halogen atom from the reaction reagent;
- and
- c) connecting the substrate bound with the linker with a first nucleic acid to obtain the patterned flow cell.
A patterned flow cell, wherein a substrate of the patterned flow cell is a flow cell substrate, the flow cell substrate comprises connection clusters arranged in an array, each connection cluster comprises a plurality of connection chains, and each connection chain comprises a first nucleic acid and a linker;
- in a direction away from the substrate of the patterned flow cell, the flow cell substrate, the linker, and the first nucleic acid are connected in sequence.
Dependencies are inferred from the claim text.