Tissue graft and processing methods
OwnerSuzanne M. TABBAA, William D. BUGBEE +1inventors
InventorsSuzanne M. TABBAA, William D. BUGBEE, Farshid GUILAK
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Abstract
Disclosed herein is a modified tissue graft (120, 200, 300, 500, 630) with improved properties for tissue integration and regeneration. The tissue graft (120, 200, 300, 500, 630) has biological properties that are enhanced by removing lipids (615) and other contaminants (e.g., blood components, micro-organisms) using enzymes, enzyme aggregates, or immobilized enzymes. This application describes the process (10) for removing bio-contaminants while maintaining optimal biological properties.
Claims18
A tissue graft comprising:
- tissue with enhanced biological properties and
- an amount of contaminants, wherein the contaminants include:
- marrow contents, blood vessels, neural tissue, adipocytes, allogeneic cells, immunogenic cells, blood cells, lipids, blood components, proteins, microorganisms or viral mRNA, and wherein the amount of contaminants is reduced by 50%, when compared to normal tissue, with enzymes, enzyme aggregates, or immobilized enzymes.
A method for removing contaminants from a tissue graft comprising:
- obtaining a tissue graft contaminated with one or more of the following contaminates:
- marrow contents, blood vessels, neural tissue, adipocytes, allogeneic cells, immunogenic cells, blood cells, lipids, blood components, proteins, microorganisms and viral mRNA, and
- removing the contaminants with a combination of enzymes, enzyme aggregates, immobilized enzymes, CO2, or cryoablation.
Dependencies are inferred from the claim text.