Immunoassay device for detecting blood type antigen-specific immunoglobulin, immunoassay method for detecting blood type antigen-specific immunoglobulin, and method for collecting blood type antigen-specific immunoglobulin

An immunoassay method for detecting blood type antigen-specific immunoglobulin, the method comprising:

• a first step of immobilizing a binding antibody having a switching peptide binding thereto to a substrate or immobilizing the binding antibody to the substrate, and then binding the switching peptide to the binding antibody, and binding a quenching substance to the binding antibody or the substrate;
• a second step of treating the binding antibody with a detection target sample solution;
• and
• a third step of irradiating light to the detection target sample solution after the treatment, and quantitatively analyzing a target antigen in the detection target sample solution based on fluorescence generated from a fluorescent label of the switching peptide released from the binding antibody,
• wherein the binding antibody includes one selected from the group consisting of a CDR3 including an amino acid sequence of SEQ ID NO 1, a CDR3 including an amino acid sequence of SEQ ID NO 2, and a CDR3 including an amino acid sequence of SEQ ID NO 3, and thus specifically binds to the blood type antigen-specific immunoglobulin,
• wherein the switching peptide includes a peptide compound having an amino acid sequence of capable selectively and reversibly binding to a Fab (Fragment antigen-binding) region of the binding antibody, and the fluorescent label binding thereto and emitting fluorescent light.

A method for collecting blood type antigen-specific immunoglobulin, the method comprising:

• adding a bead having an antibody or a peptide immobilized on a surface thereof specifically binding to the blood type antigen-specific immunoglobulin into a sample containing the blood type antigen-specific immunoglobulin to induce a reaction between the blood type antigen-specific immunoglobulin and the antibody or the peptide;
• and
• collecting the bead from the sample,
• wherein the binding antibody includes one selected from the group consisting of a CDR3 including an amino acid sequence of SEQ ID NO 1, a CDR3 including an amino acid sequence of SEQ ID NO 2, and a CDR3 including an amino acid sequence of SEQ ID NO 3, and thus specifically binds to the blood type antigen-specific immunoglobulin.