Methods for measuring interactions between proteins comprising an fc portion and particles
AssigneeNanotemper Technologies GmbH
InventorsCHRISTOPHER HEIM, WERNER STREICHER, PHILIPP BAASKE, PHILIPP SCHRAMM
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Abstract
The present invention relates to means and methods for measuring an interaction between a fluorescently labelled protein comprising an Fc portion and particles in a solution, wherein said protein comprising an Fc portion is fluorescently labelled by a Fc-binding peptide comprising a fluorescent label. Means may, e.g. be kits or systems which may also be used measuring an interaction between a fluorescently labelled protein comprising an Fc portion and particles in a solution.
Claims15
A method for measuring an interaction between a fluorescently labelled protein comprising an Fc portion and one or more particles in a solution, comprising analysing alterations in the fluorescence spectrum of said labelled protein comprising an Fc portion upon a potential interaction with said particles, wherein said protein comprising an Fc portion is fluorescently labelled by a Fc-binding peptide capable of binding to said protein comprising an Fc portion within its Fc region, said Fc-binding peptide comprising a fluorescent label, wherein the alterations in the fluorescence spectrum of said labelled protein comprising an Fc portion are analysed by microscale thermophoresis, temperature-related intensity change or spectral shift technology.
A system comprising a Fc-binding peptide comprising (i) a fluorescent label, (ii) a protein comprising an Fc portion which is to be bound by said Fc-binding peptide and (iii) a device configured to analyse alterations in the fluorescence spectrum of said labelled protein comprising an Fc portion upon a potential interaction of said labelled protein comprising an Fc portion with particles, wherein said device is configured to perform microscale thermophoresis, temperature-related intensity change or spectral shift measurement.
Dependencies are inferred from the claim text.