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AssigneeJiangsu Province Hospital the First Affiliated Hospital With Nanjing Medical University
InventorsHeng CHEN, Tao YANG, Yong GU
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A protein scaffold capable of carrying radioactive signals and application thereof in antibody detection are provided. The present disclosure successfully finds out a protein scaffold R12 capable of carrying radioactive signals, which is used for assisting part of antigens to capture corresponding antibodies; and because the method is used for carrying signals and eliminating more severe screening conditions of cross reaction in the screening process, the method is not limited to the method in application, and the method can be directly used for a technical platform which only needs to eliminate the cross reaction.
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An application of a protein scaffold in a detection kit for an anti-butyrophilin 3A1 antibody, wherein the detection kit for the anti-butyrophilin 3A1 antibody comprises a radioactive antigen protein AR12A, the radioactive antigen protein AR12A is obtained by labelling the antigen protein AR12A with S35-methionine, and a gene sequence of the antigen protein AR12A comprises a gene sequence of the protein scaffold and a gene sequence of the BTN3A1;
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