Biomarkers for predicting preterm birth due to preterm premature rupture of membranes (pprom) versus idiopathic spontaneous labor (ptl)

A method of determining probability for preeclampsia in a pregnant female, the method comprising:

• (a) detecting the presence or amount of one or more biomarkers in a biological sample obtained from a pregnant female;
• and
• (b) measuring a reversal value for said one or more biomarkers,
• wherein said reversal value predicts the probability for preeclampsia in said pregnant female in a predictive model,
• wherein detecting comprises subjecting said biological sample to:
• (i) a proteomics work-flow comprised of mass spectrometry (MS) quantification;
• or (ii) an assay that utilizes a capture agent that binds to at least one biomarker of said one or more biomarkers, and
• wherein the one or more biomarkers comprise Notum or ISM2.

A method of detecting a reversal value for one or more isolated biomarkers or a pair of isolated biomarkers in a biological sample obtained from a pregnant female, the method comprising:

• (a) detecting the presence or amount of said one or more isolated biomarkers or pair of isolated biomarkers in said biological sample;
• and
• (b) measuring a reversal value for said pair of isolated biomarkers,
• wherein detecting comprises subjecting said biological sample to:
• (i) a proteomics work-flow comprised of mass spectrometry (MS) quantification;
• or (ii) an assay that utilizes a capture agent that binds to at least one biomarker of said one or more isolated biomarkers or said pair of isolated biomarkers,
• wherein said biological sample is selected from the group consisting of whole blood, plasma, and serum, and
• wherein said one or more isolated biomarkers or said pair of isolated biomarkers comprises Notum or ISM2.