Methods for Generating Biologically Specific Chromatin Condensates and Uses Thereof

A method to distinguish nucleosome array condensates that have different chromatin states, the method comprising:

• g) performing fluorescence imaging of fluorescently labeled nucleosome array condensates to obtain fluorescence images;
• h) performing segmentation-free image quantification on the fluorescence images;
• i) computing size distributions of the nucleosome array condensates from the fluorescence images;
• j) reducing complexities of the size distributions into distinct features that discriminate between the fluorescence images to simplify quantification of the nucleosome array condensates;
• k) acquiring shape distributions for the nucleosome array condensates from the fluorescence images;
• and
• l) reducing complexities of the shape distributions into distinct features that discriminate between the fluorescence images to simplify quantification of the nucleosome array condensates.

A computer implemented method for processing fluorescence images of fluorescently labeled nucleosome array condensates, the computer performing steps comprising:

• g) receiving the fluorescence images of the fluorescently labeled nucleosome array condensates;
• h) performing segmentation-free image quantification on the fluorescence images;
• i) computing size distributions of the nucleosome array condensates from the fluorescence images;
• j) reducing complexities of the size distributions into distinct features that discriminate between the fluorescence images to simplify quantification of the nucleosome array condensates;
• k) acquiring shape distributions for the nucleosome array condensates from the fluorescence images;
• and
• l) reducing complexities of the shape distributions into distinct features that discriminate between the fluorescence images to simplify quantification of the nucleosome array condensates.