Optical multianalyte detection

An optical method for detecting the presence of a target analyte in a sample, the method comprising:

• a) moving automatically a first portion of a whole blood sample from a support pack to a first well in a disk in a detection instrument, the disk comprising dried chemistries for processing the sample, plasma separation features configured to separate whole blood into plasma, and a hematocrit channel configured to execute the hematocrit assay, the support pack comprising the whole blood sample, pipette tips, buffers, a reagent for processing the sample, reagent preparation wells, and a monolayer assay device configured to operate as a hematology slide:
• b) separating by the detection instrument plasma from the first portion of the whole blood sample in the disk while simultaneously moving reagent in the support pack for processing the plasma generated in the disk:
• c) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the disk to the support pack:
• d) diluting by the detection instrument the plasma generated from the first portion of the whole blood sample in the support pack:
• e) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the support pack to the disk:
• f) optically detecting by the detection instrument the presence or absence of said first target analyte, the detection instrument comprising a laser and at least one of a spectrophotometer, or a camera:
• further comprising moving by the detection instrument a third portion of the whole blood sample from the support pack to a second well in the disk;
• hybridizing by the detection instrument a probe to a second target analyte in the second well to form a bead-antigen-fluorescent complex;
• moving by the detection instrument the bead-antigen- fluorescent complex to the support pack;
• washing automatically by the detection instrument the bead-antigen-fluorescent complex in the support pack;
• moving the washed bead-antigen-fluorescent complex back to the disk to a third well wherein the second well and the third well are different;
• and detecting by the detection instrument the presence or absence of said second target analyte in the disk after detecting by the detection instrument the presence or absence of said first target analyte in the disk.

An optical method for detecting the presence of target analyte in a sample, the method comprising:

• a) receiving by a detection instrument a first cartridge comprising dried chemistries, plasma separation features, and a hematocrit channel;
• b) receiving by the detection instrument a second cartridge comprising the whole blood sample, pipette tips, buffers, reagent preparation wells, and a monolayer assay device;
• c) moving automatically by the detection instrument a first portion of a whole blood sample from the second cartridge to a first well in the first cartridge and moving automatically by the detection instrument a second portion of a whole blood sample from the second cartridge to a reaction well in the second cartridge;
• d) moving automatically by the detection instrument a reagent from the second cartridge to the first well in the first cartridge;
• e) and diluting the second portion of the whole blood sample in the second cartridge;
• and f optically detecting by the detection instrument the presence or absence of a first target analyte in the first portion, the instrument comprising a laser and at least one of a spectrophotometer, and a camera.

An optical method for detecting the presence of target analyte in a sample, the method comprising:

• a) moving automatically a first portion of a whole blood sample from a second cartridge to a first well in a first cartridge in a detection instrument, the first cartridge comprising dried chemistries, plasma separation features, and a hematocrit channel, the second cartridge comprising the whole blood sample, pipette tips, buffers, reagent preparation wells, and a monolayer assay device;
• b) moving reagent from the second cartridge to the first well in the first cartridge;
• c) optically detecting by the detection instrument the presence or absence of a first target analyte in the first well, the detection instrument comprising a laser and at least one of a spectrophotometer or a camera;
• (d) moving automatically a second portion of the whole blood sample from the second cartridge to a first tube in the second cartridge in a detection instrument;
• (e) moving automatically a buffer from the second cartridge to the first tube in the second cartridge;
• (f) optically detecting by the detection instrument the presence or absence of a second target analyte in the first tube.

An optical method for detecting the presence of a target analyte in a sample, the method comprising:

• a) moving automatically a first portion of a whole blood sample from a support pack to a first well in a disk in a detection instrument, the disk comprising dried chemistries for processing the sample, plasma separation features configured to separate whole blood into plasma, and a hematocrit channel configured to execute the hematocrit assay, the support pack comprising the whole blood sample, pipette tips, buffers, a reagent for processing the sample, reagent preparation wells, and a monolayer assay device configured to operate as a hematology slide:
• b) separating by the detection instrument plasma from the first portion of the whole blood sample in the disk while simultaneously moving reagent in the support pack for processing the plasma generated in the disk:
• c) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the disk to the support pack:
• d) diluting by the detection instrument the plasma generated from the first portion of the whole blood sample in the support pack:
• e) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the support pack to the disk:
• f) optically detecting by the detection instrument the presence or absence of said first target analyte, the detection instrument comprising a laser and at least one of a spectrophotometer, or a camera;
• and further comprising optically detecting by the detection instrument the presence or absence of said first target analyte in the first portion of the whole blood sample in the disk while simultaneously optically detecting by the detection instrument the presence or absence of an additional target analyte in an additional portion of the whole blood sample in the support pack.