Electrochemical measuring method using biochemical test chip

An electrochemical measuring method, comprising:

• providing a biochemical test chip including:
• an insulating substrate;
• an electrode unit, located on the insulating substrate and including a working electrode and a counter electrode;
• a first insulating septum, located on the electrode unit and having an opening at least partially exposing the electrode unit;
• a reactive layer, located at the opening and electrically connected to the electrode unit;
• and
• a second insulating septum, located on the first insulating septum, and reacting the reactive layer with a target analyte as a primary reaction,
• wherein during the primary reaction,
• the counter electrode undergoes a self-redox reaction without interfering with the primary reaction,
• the self-redox reaction allows the counter electrode capable of receiving or releasing additional electrons, and
• a current density of the counter electrode is greater than a current density of the working electrode,
• wherein the counter electrode is an anode, and a standard reduction potential of an active material of the counter electrode satisfies Es0<Em0−Ev, wherein the Es0 is the standard reduction potential of the active material, the Em0 is a standard reduction potential for concentration reaction on the working electrode, and the Ev is a potential applied by a measuring apparatus when providing measuring reaction.