Analysis method, analysis apparatus, and analysis kit for calculating concentration of target substance

An analysis apparatus for determining at least any one of:

• presence or absence of a target substance;
• and a concentration of the target substance, the analysis apparatus comprising:
• a glass vessel to be used for accommodating a reaction liquid containing:
• a sample containing the target substance;
• and a reagent that reacts with the target substance;
• a unit configured to load the sample containing the target substance into the glass vessel;
• a unit configured to load a hydrophilic polymer that reacts with a silanol group of the glass vessel into the glass vessel;
• a unit configured to load the reagent into the glass vessel after or simultaneously with the loading of the hydrophilic polymer;
• an irradiation unit configured to irradiate, as irradiation light, the glass vessel with light that is linearly polarized light;
• a measurement unit configured to measure fluorescence intensities of fluorescence of two polarized light components in a parallel direction and an orthogonal direction with respect to the linearly polarized light;
• and
• a processing unit configured to process results obtained by the measurement unit, to thereby determine at least any one of:
• the presence or absence of the target substance;
• and the concentration of the target substance in the reaction liquid,
• wherein the reagent includes a luminescent reagent having a hydrophilic surface, and the luminescent reagent includes a luminescent particle.

An analysis kit to be used in an analysis method for determining at least any one of:

• presence or absence of a target substance;
• and a concentration of the target substance based on polarization anisotropy, the analysis kit comprising:
• a reagent that reacts with the target substance;
• a glass vessel to be used for accommodating a reaction liquid containing:
• a sample containing the target substance;
• and the reagent;
• and
• a hydrophilic polymer that reacts with a silanol group of the glass vessel,
• wherein the reagent includes a luminescent reagent having a hydrophilic surface, and the luminescent reagent includes a luminescent particle.