Assessment and diagnosis myxomatous mitral valve disease using a microrna assay
AssigneeMI:RNA Ltd.
InventorsEve HANKS, Robert COULTOUS
Life of this patent
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Abstract
A method of assessing expression profiles of miRNA markers using predictive classification models to differentially diagnosing MMVD patients from healthy controls or DCM patients from healthy controls. Additionally, an assessment of the same method to discriminate pre-clinical from clinical MMVD or DCM patients. Also provided is a method of differentially diagnosing MMVD patients from DCM patients or from healthy controls.
Claims20
A method in a computer-implemented system comprising at least one processor and at least one memory, the at least one memory comprising instructions executed by the at least one processor to cause the at least one processor to implement one or more predictive classification models to assess and differentially diagnose a cardiac disease or conditions in a subject, comprising the steps of:
- (a) obtaining a sample from the subject;
- (b) determining a level of expression of each of a plurality of miRNA molecules within the sample;
- (c) applying the one or more predictive classification models to the expression of each of a plurality of miRNA molecules;
- (d) using the predictive classification models to differentially classify the diseased state of the cardiac disease or condition in the subject;
- and
- (e) using the classification of the diseased state of the cardiac disease or condition to predict the disease condition of the subject;
- wherein the cardiac condition is myxomatous mitral valve disease (MMVD), mitral regurgitation (MR), dilated cardiomyopathy disease, or hypertrophic cardiomyopathy (HCM).
A method of selecting a panel for use in disease diagnosis comprising the steps of:
- (a) obtaining a sample from the subject;
- (b) determining a level of expression of each of a plurality of miRNA molecules within the sample, having at least 99% sequence identity to SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, and 15;
- (c) using a computer-implemented system comprising at least one processor and at least one memory, the at least one memory comprising instructions executed by the at least one processor to cause the at least one processor to apply machine learning algorithms to generate one or more predictive classification models;
- (d) applying the one or more predictive classification models to the expression of each of a plurality of miRNA molecules;
- and
- (e) using the predictive classification models to diagnose a cardiac disease in the subject;
- wherein the cardiac condition is myxomatous mitral valve disease (MMVD), mitral regurgitation (MR), dilated cardiomyopathy disease, or hypertrophic cardiomyopathy (HCM).
Dependencies are inferred from the claim text.