Expanding the Molecular Processing and Biosensing Capabilities of a Single-Construct Quantum Dot-Based Biosensor By Selectively Controlling Energy Transfer Pathways

A modular energy transfer platform comprising:

• a CdSe/ZnS core-shell quantum dot (QD) scaffold having four additional components bound thereto, the additional components comprising terbium, ruthenium, AlexaFluor 647, and Cy5.5, wherein
• each of the additional components are bound to the QD scaffold via an arm, each arm comprising a peptide-PNA strand comprising (1) a peptide nucleic acid (PNA) comprising a PNA sequence and (2) a peptide comprising a polyhistidine sequence effective to bind the peptide-PNA strand to the QD;
• and
• the arm comprising ruthenium features the ruthenium conjugated to the PNA sequence via a modified peptide derivative.