Barcoded molecular standards

A calibrator double-stranded DNA molecule, comprising:

• two non-contiguous regions each present in different strands of the calibrator molecule, wherein the two non-contiguous regions are respectively complementary on opposite strands to a pair of primers, the pair of primers being complementary to a first strand and a second strand of a DNA analyte at respective ends of a region to be amplified of the DNA analyte;
• a calibrator region that is distinct from the region to be amplified of the DNA analyte, wherein the calibrator region is between the two non-contiguous regions;
• a unique molecular barcode that is unique to a specific patient sample, a specific set of patient samples, a specific project, a specific batch, or a specific lot;
• and
• a pair of priming sites distal to and surrounding the two non-contiguous regions, wherein the pair of priming sites is not present in the DNA analyte, the pair of priming sites being capable of priming amplification of the calibrator molecule without priming amplification of the DNA analyte.