Method and Device for Detecting Presence, Concentration, and Antibiotic Resistance of Bacteria in Urine Samples

Abstract

An automated system that determines the presence of bacteria in a urine sample, concentration of the bacteria, and the bacteria's resistance to a panel of antibiotics, in around one hour instead of days. An automated assay to detect cell viability in mammalian cell cultures using reduction of alamarBlue through oxidation by NADH to NAD+. The assay is conducted within a fluidic chip, wherein a sample is mixed with alamarBlue, a weakly fluorescent indicator dye. Its rate of conversion to a highly fluorescent compound is monitored using a kinetics fluorimeter for 15 minutes, and metabolic activity is shown by an increase in fluorescence. A sample with live bacteria displays a consistent and rapid increase in fluorescence, whereas the negative control exhibits little to no increase over time.

Dependencies are inferred from the claim text.