Method and device for detecting at least one microorganism according to the staining kinetics thereof, and detection support
AssigneeRedberry
InventorJoseph PIERQUIN
Life of this patent
Estimating the term…
Abstract
A method for detecting, on a detection support, at least one microorganism present in a sample to be analysed and revealed by means of at least one cell marker, includes a step of taking of an image (I0, I′0) before a step of controlled release of the marker and bringing this marker into contact with said microorganism, and at least one step of taking of an image (Ii) following this contact step, so as to detect the microorganism according to the change in the staining kinetics thereof, by means of a comparative analysis between the different images that have been taken.
Claims32
A method for detecting, on a solid detection support, at least one microorganism, of bacteria, yeast or mould type, present in a sample to be analysed and revealed by means of at least one cell marker, said method comprising at least the steps, in a sequential order, of:
- a) depositing said sample to be analysed on said solid detection support;
- b) illuminating said detection support at least once with light radiation capable of revealing said at least one cell marker;
- c) acquiring at least one image (I0) in a first phase P0 of said sample on at least one portion of said detection support by means of an optical imaging device targeting a field of view that is at least 10 mm long by at least 10 mm wide;
- d) carrying out a controlled release of said at least one cell marker, through the detection support, to bring said marker into contact with said microorganism;
- e) acquiring, by means of said optical device, an image (Ii) (i≥1) of each portion of the detection support of which an image was acquired at P0 in at least one subsequent phase Pi after the step of the controlled release of said marker, and detecting said at least one microorganism according to a change in staining kinetics of said microorganism;
- and
- f) carrying out a comparative analysis, per portion of the detection support, between said image (I0) acquired at P0 and each image (Ii) acquired in at least one subsequent phase Pi and drawing a conclusion as regards the presence or absence of at least one microorganism in said sample.
A method for detecting, on a solid detection support, at least one microorganism, of bacteria, yeast or mould type, present in a sample to be analysed and revealed by means of at least one cell marker, said method comprising at least the steps, in a sequential order, of:
- a1) depositing said sample to be analysed on said solid detection support;
- b1) illuminating locally said detection support by light radiation capable of revealing said at least one cell marker;
- c1) simultaneously with the illumination step b1), acquiring at least one image (I0, I′0) in a first phase P0 of said sample on at least one portion of said detection support by means of an optical imaging device targeting a field of view that is at least 10 mm long by at least 10 mm wide;
- d1) carrying out a controlled release of said at least one cell marker, through the detection support, to bring said marker into contact with said microorganism;
- e1) within a lapse of time after step d1) of the controlled release of said cell marker, illuminating locally said detection support with said light radiation capable of revealing said at least one cell marker;
- f1) simultaneously with the illumination step e1), acquiring an image (I1, I′1), by means of said optical device, of each portion of the detection support of which an image was acquired at P0 in at least one subsequent phase P1 after the step of the controlled release of said marker;
- g1) re-illuminating locally, at least once, said detection support with said light radiation;
- h1) simultaneously with the illumination step g1), acquiring an image (I2, I′2), by means of said optical device, of each portion of the detection support of which an image was acquired at P0 and at P1 in at least one second subsequent phase P2 in order to detect said at least one microorganism according to the change in the staining kinetics of said microorganism;
- and
- i1) carrying out a comparative analysis, per portion of the detection support, between said image (I0, I′0) acquired at P0 and said images (I1, I′1;
- I2, I′2) acquired in at least two subsequent phases P1 and P2 and drawing a conclusion as regards the presence or absence of at least one microorganism in said sample.
Dependencies are inferred from the claim text.