Recombinant of Hydrogenophilus Bacterium with Enhanced Ability to Produce Valine
AssigneeUtilization of Carbon Dioxide Institute Co., Ltd.
InventorsHideaki YUKAWA, Naoto OHTANI
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Abstract
A transformant obtained by introducing (a) a lactate dehydrogenase gene and/or (b) a malate/lactate dehydrogenase gene into a Hydrogenophilus bacterium efficiently produces lactic acid through use of carbon dioxide as a sole carbon source. Parageobacillus thermoglucosidasius ldh gene, Geobacillus kaustophilus ldh gene and Thermus thermophilus ldh gene of lactate dehydrogenases, and Thermus thermophilus mldh gene and Meiothermus ruber mldh-1 and mldh-2 genes of malate/lactate dehydrogenases are preferable in that they have good lactic acid production efficiency.
Claims6
A transformant obtained by introducing a lactate dehydrogenase gene and/or a malate/lactate gene into a Hydrogenophilus bacterium
- wherein the lactate dehydrogenase gene comprises at least one selected from the group consisting of:
- DNA which consists of a base sequence having 90% or more identity to SEQ ID NO:
- 1, 2 or 3;
- DNA which encodes a polypeptide consisting of an amino acid sequence having 90% or more identity to SEQ ID NO:
- 4, 5 or 6;
- and
- DNA which encodes a polypeptide consisting of an amino acid sequence having a deletion, substitution, or addition of 1 to 5 of amino acids in an amino acid sequence of SEQ ID NO:
- 4, 5 or 6,
- wherein the malate/lactate dehydrogenase gene comprises at least one selected from the group consisting of
- DNA which consists of a base sequence having 90% or more identity to SEQ ID NO:
- 7, 8 or 9;
- DNA which encodes a polypeptide consisting of an amino acid sequence having 90% or more identity to SEQ ID NO:
- 10, 11 or 12;
- and
- DNA which encodes a polypeptide consisting of an amino acid sequence having a deletion, substitution, or addition of 1 to 5 of amino acids in an amino acid sequence of SEQ ID NO:
- 10, 11 or 12.
Dependencies are inferred from the claim text.