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US 2025/0084392 A1Published application
Strategies for direct recruitment of repair templates to crispr nucleases
AssigneeInscripta, Inc.
InventorsBrian Chaikind, Christopher Lim
Life of this patent
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Abstract
This invention relates to compositions of matter, methods and instruments for directly recruiting repair templates to CRISPR nucleases to stimulate homology-directed repair. Molecular “tethers” are described which result in an increase in the local concentration of repair templates at the site of the double-strand break made by a nuclease, thereby enhancing the rate of homology directed repair and suppressing undesired edits.
Claims20
A system comprising:
- (i) a fusion polypeptide comprising first and second orthogonal nucleases and further comprising or coupled to a recruiting moiety, (ii) a repair template comprising or coupled with a binding moiety, and optionally (iii) one or more guide RNAs;
- wherein the recruiting moiety recognizes the binding moiety and forms a binding pair.
A system comprising:
- (i) an RNA-guided nuclease, (ii) a guide RNA comprising or coupled to a recruiting moiety, and (iii) a repair template molecule comprising or coupled with a binding moiety;
- wherein the recruiting moiety recognizes the binding moiety and forms a binding pair.
Dependencies are inferred from the claim text.