Chimeric poxvirus compositions and uses thereof
AssigneeCity of Hope
InventorsYuman Fong, Nanhai Chen
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Abstract
Provided herein are, inter alia, viral compositions and methods of using the same. The viral compositions provided include, inter alia, therapeutically effective amounts of a chimeric poxvirus and are particularly useful for methods of treating cancer. The chimeric poxviruses provided herein may further include transgenes.
Claims37
A chimeric poxvirus comprising a nucleic acid sequence having a sequence identity of at least 95%1 to positions 5,014 to 185,366 of SEQ ID NO:1 or to SEQ ID NO:2, wherein said nucleic acid sequence further comprises:
- (i) one or more anti-cancer nucleic acid sequences inserted into said nucleic acid sequence;
- or
- (ii) a detectable moiety-encoding nucleic acid sequence inserted into said nucleic acid sequence.
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A method of forming a chimeric poxvirus, said method comprising:
- (i) infecting a cell with at least two poxvirus strains selected from the group consisting of cowpox virus strain Brighton, raccoonpox virus strain Herman, rabbitpox virus strain Utrecht, vaccinia virus strain WR, vaccinia virus strain IHD, vaccinia virus strain Elstree, vaccinia virus strain CL, vaccinia virus strain Lederle-Chorioallantoic, vaccinia virus strain AS, orf virus strain NZ2 and pseudocowpox virus strain TJS;
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- (ii) allowing said at least two poxvirus strains to replicate, thereby forming said chimeric poxvirus.
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Dependencies are inferred from the claim text.