Cell monolayer blocking agents and uses thereof

A method for preparing target cells attached to a coated surface, comprising the steps of:

• providing a surface coated with a polypeptide for attachment of target cells;
• providing target cells;
• providing an NHS ester reagent;
• contacting the target cells with the coated surface and allowing the target cells to attach to the coated surface;
• blocking the coated surface with attached target cells with the NHS ester agent.

A method for preparing target cells attached to a coated surface, comprising the steps of:

• providing a surface for attachment of target cells;
• providing target cells;
• provide a polypeptide for coating;
• providing an NHS ester reagent;
• coating the surface with a polypeptide to provide a coated surface;
• contacting the target cells on the coated surface and allowing the target cells to attach to the coated surface;
• blocking the target cells attached to the coated surface with the NHS ester agent.