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US 2025/0084367 A1Published application
Systems and methods for growing cells in vitro
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorYaakov NAHMIAS
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Abstract
A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.
Claims7
A method of generating a spontaneously immortalized fibroblast, comprising:
- (a) culturing avian embryo cells in the presence of a serum-containing medium under adherent culture conditions to thereby obtain chicken embryonic fibroblasts,
- (b) passaging said avian embryonic fibroblasts for at least 10-12 passages in said serum-containing medium under said adherent conditions until culture collapse, wherein said culture collapse is characterized by senescence and/or death of at least 90% of said avian embryonic fibroblasts,
- (c) isolating at least one colony which survived said culture collapse in said serum-containing medium for at least additional 20 passages,
- thereby generating the spontaneously immortalized fibroblast.
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Dependencies are inferred from the claim text.