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AssigneeTrustees of Tufts College
InventorsDavid L. Kaplan, Andrew Stout
Estimating the term…
A serum-free culture media for expansion of the number of cells and related method of use are disclosed. The disclosed media can be for use in cultured food applications. The disclosed media can include a baseline serum-free and animal component-free culture media and a plant protein composition. The plant protein composition can comprise unhydrolyzed plant proteins. The plant protein composition can comprise plant proteins that are 3 kDa in size or greater. The plant protein composition can be present in the serum-free cell culture media at a final concentration of 0.05 g/L to 1 g/L. The baseline media, in use, provides a baseline growth capability for expansion of the numbers of muscle satellite cells for use in cultured food applications. The disclosed media, in use, provides an improved growth capability for expansion of the number of muscle satellite cells. Methods of making and using the disclosed media are also disclosed.
A serum-free cell culture media for expansion of cells, the media comprising:
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A method of making a serum-free cell culture media, the method comprising adding a plant protein composition at a final concentration of 0.05 g/L to 50 g/L in the serum-free cell culture media to a basal culture media, thereby producing the serum-free cell culture media.
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A method of expanding the numbers of or differentiating cells, the method comprising:
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Dependencies are inferred from the claim text.