Microfluidic cell culture

A microfluidic device comprising:

• one or more discrete microfluidic circuits each configured to hold a fluid, the microfluidic circuit defined by an enclosure, the enclosure comprising:
• a base comprising a first material;
• a microfluidic circuit structure comprising a second material;
• and
• a cover comprising a third material,
• wherein inner surfaces of the base, the microfluidic circuit structure, and the cover form inner surfaces facing an interior of at least one growth chamber, the inner surfaces facing the interior of the at least one growth chamber including a conditioned surface covalently attached thereto, the conditioned surface configured to support cell growth, viability, portability, or any combination thereof within the microfluidic device,
• wherein the conditioned surface includes a cleavable moiety that is configured to permit disruption of the at least one conditioned surface to facilitate portability of one or more biological cells.

A method of culturing at least one biological cell in a microfluidic device, the microfluidic device comprising:

• one or more discrete microfluidic circuits each configured to hold a fluid, the microfluidic circuit defined by an enclosure, the enclosure comprising:
• a base comprising a first material;
• a microfluidic circuit structure comprising a second material;
• and
• a cover comprising a third material,
• wherein inner surfaces of the base, the microfluidic circuit structure, and the cover form inner surfaces facing an interior of at least one growth chamber, the inner surfaces facing the interior of the at least one growth chamber including a conditioned surface covalently attached thereto, the conditioned surface configured to support cell growth, viability, portability, or any combination thereof within the microfluidic device,
• wherein the conditioned surface includes a cleavable moiety that is configured to permit disruption of the at least one conditioned surface,
• wherein the method comprises:
• introducing the at least one biological cell into the at least one growth chamber;
• incubating the at least one biological cell for a period of time at least long enough to expand the at least one biological cell to produce a colony of biological cells;
• and
• cleaving one or more cleavable moieties of the conditioned surface after the incubating, thereby facilitating export of one or more biological cells of the colony of biological cells out of the at least one growth chamber and into a flow region of the microfluidic device.

A system for culturing one or more biological cells comprising:

• a microfluidic device comprising:
• one or more discrete microfluidic circuits each configured to hold a fluid, the microfluidic circuit defined by an enclosure, the enclosure comprising:
• a base comprising a first material;
• a microfluidic circuit structure comprising a second material;
• and
• a cover comprising a third material,
• wherein inner surfaces of the base, the microfluidic circuit structure, and the cover form inner surfaces facing an interior of at least one growth chamber, the inner surfaces facing the interior of the at least one growth chamber including a conditioned surface covalently attached thereto, the conditioned surface configured to support cell growth, viability, portability, or any combination thereof within the microfluidic device,
• wherein the conditioned surface includes a cleavable moiety that is configured to permit disruption of the at least one conditioned surface to facilitate portability of one or more biological cells;
• and
• a temperature control configured to modulate a temperature of the at least one conditioned surface within a predetermined temperature range conducive for maintaining functional cells.