Physiologically-relevant, serum-free in vitro angiogenesis platform

A system for a physiologically-relevant, serum-free in vitro angiogenesis platform, comprising:

• an incubator operably configured for housing a cell media;
• a peristaltic pump for moving the cell media;
• a microscope for conducting microscopy of the cell media;
• and
• a cell chamber having one or more perfusable channels, a viewing window for observation with the microscope, and one or more ports in a sidewall of the cell chamber for introducing one or more concentrations of nanoparticles;
• wherein endothelial cells line the one or more perfusable channels.

A method for physiologically-relevant, serum-free in vitro angiogenesis, comprising:

• providing a cell chamber having one or more perfusable channels, a viewing window for microscopy observation, and one or more ports in a sidewall of the cell chamber;
• introducing cell media into the cell chamber;
• introducing one or more concentrations of nanoparticles into the cell chamber via the one or more ports in the sidewall of the cell chamber;
• and
• circulating cell media through the cell chamber.