Antibody specific for mucin-1 and methods of use thereof

A nucleic acid encoding a heavy chain variable domain (VH), a light chain variable domain (VL), or both, of an antibody that specifically binds to Mucin-1 (MUC-1),

• wherein the VH comprises a heavy chain complementarity determining region 1 (HCDR1), a heavy chain complementarity determining region 2 (HCDR2), and a heavy chain complementarity determining region 3 (HCDR3) as set forth in a VH comprising the amino acid sequence of SEQ ID NO:
• 1, and
• wherein the VL comprises a light chain complementarity determining region 1 (LCDR1), a light chain complementarity determining region 2 (LCDR2), and a light chain complementarity determining region 3 (LCDR3) as set forth in a VL comprising the amino acid sequence of any one of SEQ ID NOs:
• 2, 3, or 4.

A cell comprising:

• a first nucleic acid encoding a VH of an antibody that specifically binds to MUC-1, wherein the VH comprises an HCDR1, an HCDR2, and an HCDR3 as set forth in a VH comprising the amino acid sequence of SEQ ID NO:
• 1;
• and
• a second nucleic acid encoding a VL of the antibody, wherein the VL comprises a LCDR1, a LCDR2, and a LCDR3 as set forth in a VL comprising the amino acid sequence of any one of SEQ ID NOs:
• 2, 3, or 4.

A fusion protein, comprising:

• a VH, a VL, or both, of an antibody that specifically binds to MUC-1;
• fused to
• a heterologous amino acid sequence,
• wherein the VH comprises an HCDR1, an HCDR2, and an HCDR3 as set forth in a VH comprising the amino acid sequence of SEQ ID NO:
• 1, and
• wherein the VL comprises a LCDR1, a LCDR2, and a LCDR3 as set forth in a VL comprising the amino acid sequence of any one of SEQ ID NOs:
• 2, 3, or 4.