Nucleic acid molecule encoding car and vector comprising the same, immune cell including car and pharmaceutical composition comprising the cell, method for improving cytotoxic activity, and method for producing immune cell including car

An immune cell comprising a chimeric antigen receptor comprising an extracellular domain, a transmembrane domain and an intracellular domain, wherein

• the extracellular domain comprises an antigen binding region comprising a light chain variable region and a heavy chain variable region,
• at least three amino acid residues of framework region 3 of the light chain variable region as defined by the Chothia method are acidic amino acid residues, and
• the at least three amino acid residues comprises at least three selected from the group consisting of an amino acid residue at position 60, an amino acid residue at position 63, an amino acid residue at position 65, an amino acid residue at position 67, an amino acid residue at position 70, an amino acid residue at position 72, an amino acid residue at position 74, and an amino acid residue at position 76 in the light chain variable region.

A method for improving cytotoxic activity of an immune cell comprising a chimeric antigen receptor,

• wherein the chimeric antigen receptor comprises an extracellular domain, a transmembrane domain and an intracellular domain, and the extracellular domain comprises an antigen binding region comprising a light chain variable region and a heavy chain variable region,
• the method comprising
• changing at least three amino acid residues of framework region 3 of the light chain variable region defined by the Chothia method to acidic amino acid residues to improve cytotoxic activity of an immune cell including the chimeric antigen receptor as compared to a chimeric antigen receptor before changing the at least three amino acid residues to acidic amino acid residues.