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US 2025/0084175 A1Published application
GENERATION OF CHIMERIC ANTIGEN RECEPTOR mRNA MOLECULES FOR EXPRESSION IN PRIMARY NK CELLS
AssigneeImmunityBio, Inc.
InventorFereshteh PARVIZ
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Abstract
The invention relates to novel chimeric antigen (CAR) mRNA molecules, the methods of generating the molecules and methods of treating cancer with the molecules.
Claims27
A chimeric antigen receptor (CAR) comprising:
- a T7 promoter, a spacer sequence, a signal peptide, an antigen binding domain, a hinge region, a transmembrane (TM) domain, and an intracellular domain;
- wherein the signal peptide comprises a cluster of differentiation 64 (CD64) and/or an IgG heavy chain variable gene (IgGHv) signal peptide;
- and
- wherein the antigen binding domain binds to an antigen selected from the group consisting of cluster of differentiation 19 (CD19), B-cell maturation antigen (BCMA), B7 homolog 4 (B7H4), SARS-CoV-2 spike, and cluster of differentiation 30 alpha (CD30α).
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A modified primary Natural Kill (NK) cell modified with an RNA molecule comprising one or more nucleic acids of a T7 promoter, a spacer sequence, a signal peptide sequence portion, an antigen binding domain sequence portion, a hinge region sequence portion, a transmembrane (TM) domain sequence portion and an intracellular domain sequence portion;
- wherein the signal peptide sequence comprises a sequence encoding cluster of differentiation 64 (CD64) and/or an IgG heavy chain variable gene (IgGHv);
- wherein the antigen binding domain comprises a sequence encoding an antigen binding portion that binds to an antigen selected from the group consisting of cluster of differentiation 19 (CD19), B-cell maturation antigen (BCMA), B7 homolog 4 (B7H4), SARS-CoV-2 spike, and cluster of differentiation 30 alpha (CD30α);
- and
- wherein the nucleic acid sequences are operably linked to each other as a single polynucleotide.
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Dependencies are inferred from the claim text.