Method for establishing pluripotent stem cells bearing genes encoding antigen specific t cell receptor

An in vitro method for inducing T cells expressing a single T cell receptor for a cell-based immunotherapy, comprising the steps of:

• (1) providing human pluripotent stem cells;
• (2) introducing re-arranged genes encoding an antigen specific T cell receptor into the pluripotent stem cells;
• and
• (3) inducing T cells from the pluripotent stem cells obtained in step (2), thereby obtaining T cells expressing said antigen specific T cell receptor as the single T cell receptor, wherein none of the genes encoding Rag 1 and Rag 2 in the pluripotent stem cells is knocked out.