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US 2025/0084121 A1Published application
Bioreactive compositions and methods of use thereof
AssigneeThe Regents of the University of California
InventorsLei Wang, Nanxi Wang
Life of this patent
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Abstract
Provided herein are, inter alia, non-toxic, bioreactive unnatural amino acids and methods of using same.
Claims20
A pyrrolysyl-tRNA synthetase having at least 90% sequence identity to SEQ ID NO:1;
- wherein SEQ ID NO:1 comprises isoleucine at a position corresponding to position 302 of SEQ ID NO:1, threonine at a position corresponding to position 346 of SEQ ID NO:1, isoleucine at a position corresponding to position 348 of SEQ ID NO:1, leucine at a position corresponding to position 384 of SEQ ID NO:1, and lysine at a position corresponding to at position 417 of SEQ ID NO:1.
A method of forming a protein comprising a fluorosulfate-L-tyrosine in a cell in vivo, the method comprising contacting in the cell:
- (a) a pyrrolysyl-tRNA synthetase, and
- (b) a fluorosulfate-L-tyrosine having the formula:
- thereby producing the protein comprising the fluorosulfate-L-tyrosine, wherein the protein comprising the fluorosulfate-L-tyrosine has an amino acid side chain of the structure:
Dependencies are inferred from the claim text.