Fluorinated nucleotides and uses thereof

A nucleotide analogue comprising a nucleotide, wherein said nucleotide comprises (i) a detectable label attached to said nucleotide via a covalent linker, L2, comprising —SS—;

• and (i) a cleavable moiety attached to the 3′ oxygen of the nucleotide, wherein the cleavable moiety comprises
• wherein
• the symbol “----” is a non-covalent bond;
• L3 is a covalent linker;
• L4 is a bond, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene;
• R4 is substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
• R5 is a detectable label, an anchor moiety, or affinity anchor moiety;
• R12 is a complementary affinity anchor moiety binder;
• R13 is a detectable label;
• X1 is halogen or —N3;
• and
• X2 is hydrogen, halogen, —N3, or —CN.

A nucleic acid polymerase complex comprising:

• a polymerase;
• a primer;
• and
• a nucleotide analogue;
• wherein said polymerase is bound to (i) the nucleotide analogue and (ii) the primer,
• wherein said nucleotide analogue is bound to streptavidin via a first covalent linker, and
• wherein said streptavidin is bound to a fluorophore via a second covalent linker.