Pipette guidance in multiple-well plate patch-clamp

A patch-clamp system, said patch-clamp system comprising a patch-clamp plate and a manifold,

• wherein said patch-clamp plate comprises:
• a plurality of intracellular (IC) inlets arranged in a first array, wherein each IC inlet is in fluid connection with an IC chamber;
• and
• a plurality of extracellular (EC) inlets arranged in a second array, wherein each EC inlet is in fluid connection with an EC chamber;
• a patch-clamp substrate arranged between each EC chamber and each IC chamber, each patch-clamp substrate comprising at least one patch-clamp hole, said patch-clamp hole extending through said patch-clamp substrate and providing a fluid connection between said EC and said IC chambers;
• wherein each IC chamber comprises an IC electrode;
• and each EC chamber comprises an EC electrode;
• wherein said manifold comprises:
• a plurality of first pipetting channels arranged in a third array,
• optionally, a plurality of second pipetting channels arranged in a fourth array,
• wherein the manifold is moveable relative to the patch-clamp plate between a first position, in which each of the first pipetting channels, or each of the second pipetting channels, of said manifold are configured to align with one IC inlet of said patch-clamp plate,
• and a second position in which each of the first pipetting channels, or each of the second pipetting channels, of said manifold are configured to align with one EC inlet of said patch-clamp plate.