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US 2025/0082797 A1Published application
Methods for In Vivo Tracking of Cells
OwnerReinier Hernandez, Anatoly Pinchuk +4inventors
InventorsReinier Hernandez, Anatoly Pinchuk, Liudmila Lambert Lepesevich, Zachary Rosenkrans, Anna Thickens, Nathan Clemons
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Abstract
Methods of radiolabeling of cell surface glycans for in vivo cell tracking and imaging as well as populations of radiolabeled cells and uses thereof are disclosed.
Claims22
A method of labeling a cell for in vivo tracking, comprising:
- (a) generating an aldehyde group on a cell surface glycan of a cell via reaction with an oxidizing agent;
- and
- (b) reacting the aldehyde group on the cell surface glycan with an aldehyde-reactive bifunctional chelator,
- wherein the bifunctional chelator reacts with the aldehyde group on the cell surface glycan to ligate the bifunctional chelator to the cell surface glycan to form a labeled cell.
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A method of generating a population of radiolabeled cells for in vivo tracking, comprising:
- (a) generating aldehyde groups on cell surface glycans of a population of cells via reaction with an oxidizing agent;
- (b) reacting the aldehyde groups on the cell surface glycans with an aldehyde-reactive bifunctional chelator to ligate the bifunctional chelator to the cell surface glycans;
- and
- (c) conjugating a radionuclide to the bifunctional chelator to generate a radionuclide labeled cell population.
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A radionuclide labeled cell, comprising:
- a cell surface glycan ligated with a bifunctional chelator,
- wherein a radionuclide is conjugated to the bifunctional chelator.
Dependencies are inferred from the claim text.