Method and System for Enhanced Fluorescence Tissue and Cancer Margin Imaging

A system for analyzing a tissue, comprising:

• an excitation light unit configured to selectively produce a plurality of excitation lights, each said excitation light centered on a wavelength distinct from the centered wavelength of the other said excitation lights, wherein the plurality of excitation lights includes a plurality of first excitation lights configured to produce an autofluorescence emission from one or more biomolecules of interest present within the tissue, and a diffuse reflectance signal from the tissue, and a second excitation light is configured to produce a fluorescence emission from a fluorescent agent administered to the tissue;
• a photodetector configured to detect the autofluorescence emission and the diffuse reflectance resulting from the plurality of first excitation lights directed to the tissue and produce first signals representative of the autofluorescence emission and the diffuse reflectance, and configured to detect the fluorescence emission signal resulting from the second excitation light directed to the tissue and produce second signals representative of the fluorescence emission;
• and
• a system controller in communication with the excitation light unit, the photodetector, and a non-transitory memory storing instructions, which instructions when executed cause the system controller to:
• control the excitation light unit to sequentially produce the first excitation light and the second excitation light;
• receive and process the first signals to produce a first image representative of the first signals, and receive and process the second signals to produce a second image representative of the second signals;
• and
• analyze the tissue using the first image and the second image to identify the presence of diseased tissue within the tissue.