Method for co-cultivation and directed differentiation induction of muscle satellite cells and adipose-derived stem cells

A method for a co-cultivation and a directed differentiation induction of muscle satellite cells and adipose-derived stem cells, comprising the following steps:

• (1) the co-cultivation:
• resuspending the muscle satellite cells and the adipose-derived stem cells from a Larimichthys crocea with a Larimichthys crocea stem cell complete medium, mixing, plating, and cultivating in a biochemical incubator at 26° C. to 30° C., wherein the Larimichthys crocea stem cell complete medium is changed every two days;
• and
• (2) a co-differentiation:
• when the muscle satellite cells and the adipose-derived stem cells from the Larimichthys crocea co-cultivated in the step (1) grow to a density of 90% or more, removing the Larimichthys crocea stem cell complete medium, adding a Larimichthys crocea adipogenic differentiation medium to allow a differentiation for 7 d to 21 d, wherein the Larimichthys crocea adipogenic differentiation medium is changed every other day, and adding a Larimichthys crocea myogenic differentiation medium to allow a myogenic differentiation for 4 d to 6 d, wherein the co-differentiation lasts for 11 d to 27 d in total.