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AssigneeBforcure
InventorsMaël LE BERRE, Christophe PANNETIER, Francesco VILLA, Maryna MURLYKINA, Julien CROZZOLO
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A composition is disclosed for the amplification of nucleic acid sequences, which is intended to be mixed with a biological sample containing one or more target nucleic acid sequences and with primers specific for the target nucleic acid sequence(s), and fluorescent probes for revealing an amplification of the target nucleic acid sequence(s). The composition includes reagents and enzymes for nucleic acid application. The composition also includes a polymerizable reagent, which polymerizes under the influence of a stimulation chosen from a heat stimulation and a light stimulation, so as to form a hydrogel which locally encapsulates each target nucleic acid sequence when the composition is exposed to the stimulation. A method using said composition is also disclosed.
A masterbatch composition for the amplification of nucleic acid sequences, intended to be mixed with a biological sample containing one or more target nucleic acid sequence(s) and specific primers of the target nucleic acid sequence(s), as well as fluorescent probes for revealing an amplification of the target nucleic acid sequence(s), the composition comprising:
Dependencies are inferred from the claim text.