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US 2025/0064095 A1Published application
Edible myceliated composition
OwnerPablo Ignacio Sanchez Rey, Juan Pablo De Giacomi +1inventors
InventorsPABLO IGNACIO SANCHEZ REY, JUAN PABLO DE GIACOMI, FERNANDO MORO CORDOBÉS
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Abstract
A multi-step process for the preparation of a protein myceliated composition, in which a particular substrate comprising cereals and legumes is colonized with the mycelia of a filamentous fungus. In the process solid fermentation of the substrate is carried out. The process provides a composition that resembles actual meat and that can be used as meat analogue.
Claims20
A process for the preparation of an edible myceliated composition comprising the following steps:
- (i) Preparing a sterilized liquified inoculum of a filamentous fungal culture, preferably of a lignocellulolytic fungus by:
- (1) adding under sterile conditions to a sterilized cereal and optionally legume-containing substrate, said substrate comprising at least oat, an amount of the filamentous fungal culture, and let the mixture ferment until the substrate is colonized with hyphae forming a mycelium network to obtain a fermented substrate;
- (2) adding a sterilized isosmotic liquid to the fermented substrate in (1), and liquefying by homogenization the mixture of (2) to obtain the sterilized liquified inoculum;
- (ii) Preparing a sterilized fermentable substrate, said substrate comprising one or more cereals including oat and rice, and one or more legumes or a textured isolated protein of these legumes, by hydrating the substrate and disposing it in a container adapted to allow exchange of gases, and sterilizing the container with the substrate;
- (iii) Inoculating in sterile conditions the sterilized fermentable substrate of step (ii) with the liquified inoculum of (i);
- and
- (iv) Culturing the sterilized fermentable substrate with the liquified inoculum for a time to allow the growth of hyphae and formation of a mycelial network within the fermented substrate to obtain an edible myceliated composition.
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Dependencies are inferred from the claim text.