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US 2025/0059550 A1Published application
Constructs and methods for increased expression of polypeptides
OwnerPavan Reddy REGATTI, Ramesh Venkat MATUR +2inventors
InventorsPavan Reddy REGATTI, Ramesh Venkat MATUR, Narender Dev MANTENA, Mahima DATLA
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Abstract
The present invention relates to field of protein expression. It provides expression constructs and methods for increased expression of recombinant proteins. More particularly, it provides constructs and methods for enhanced expression of Lira-peptide in a recombinant host cell.
Claims25
An expression cassette for expression of a protein of interest, wherein the said expression cassette comprises of:
- a) polynucleotide encoding T7 leader polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1;
- b) a polynucleotide encoding expression tag polypeptide comprising of an amino acid sequence selected from the group comprising of SEQ ID NO:
- 2-10;
- c) a polynucleotide encoding a cleavable peptide linker;
- and
- d) a polynucleotide encoding the protein of interest,
- wherein the said polynucleotide sequences of the expression cassette are operably linked to a promoter.
5 more elements
An expression cassette for expression of lira-peptide, wherein the said expression cassette comprises of:
- a) a polynucleotide encoding T7 leader polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1;
- b) a polynucleotide encoding expression tag polypeptide comprising of an amino acid sequence selected from the group comprising of SEQ ID NO:
- 2-10;
- c) a polynucleotide encoding a cleavable peptide linker;
- and
- d) a polynucleotide encoding lira-peptide comprising the amino acid sequence as set forth in SEQ ID NO:
- 12 or a functional variant thereof,
- wherein the said polynucleotide sequences of the expression cassette are operably linked to a promoter.
6 more elements
A fusion polypeptide comprising of:
- a) a T7 leader polypeptide comprising the amino acid sequence of SEQ ID NO:1;
- b) an expression tag polypeptide having an amino acid sequence selected from the group comprising of SEQ ID NOs:
- 2-10;
- and
- c) a cleavable peptide linker;
- fused to the amino-terminal of a protein of interest to obtain the fusion polypeptide.
3 more elements
Dependencies are inferred from the claim text.