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AssigneeMiruku Limited
InventorsAlejandra ACEVEDO FANI, Harjinder SINGH, Mai Shamir ASOR, Oded SHOSEYOV, Miron ABRAMSON
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The invention provides modified dairy proteins in which at least one potentially phosphorylated amino acid has been replaced with a negatively charged amino acid. The modified dairy proteins exhibit at least one of: an improved capacity to binding calcium, an improved capacity to form a micelle, an altered isoelectric point (pl), altered thermal stability and an altered zeta potential, when expressed in a heterologous expression system, relative to that of the unmodified dairy protein when it is recombinantly expressed in the same heterologous expression system. The invention also provides cells, tissues, plants, plant parts and seeds, expressing the modified dairy proteins. The invention also provides methods for the production and use of the modified proteins. The invention also provides food or beverage products or ingredients comprising the modified dairy proteins.
A modified dairy protein in which at least one potentially phosphorylated amino acid has been replaced with a negatively charged amino acid.
The modified dairy protein of any preceding claim in which the negatively charged amino acid is selected from aspartate (D) and glutamate (E).
The modified dairy protein of any preceding claim which when recombinantly expressed in a heterologous expression system, exhibits at least one of the following:
The modified dairy protein of any preceding claim which when recombinantly expressed in a heterologous expression system, exhibits at least one of the following:
The modified dairy protein of any preceding claim which apart from the modifications described is otherwise the same in sequence as the unmodified dairy protein.
A polynucleotide encoding the modified dairy protein of any preceding claim
A method for producing a modified dairy protein of any preceding claim, the method comprising expressing a polynucleotide encoding the modified dairy protein in a host cell, plant cell, organism, plant, plant part, plant tissue, propagule, progeny, or seed.
The method of any preceding claims comprising the step of purifying the modified dairy protein from the host cell, plant cell, organism, plant, plant part, plant tissue, propagule, progeny, or seed.
A modified dairy protein produced by the method of any preceding claim.
A food or beverage product or ingredient comprising one or more modified dairy protein of any preceding claim or produced by the method of any preceding claim.
A method for producing a food or beverage product or ingredient, the method including the step of processing or incorporating the modified dairy protein of any preceding claim, or produced by the method of any preceding claim, into food or beverage product or ingredient.
Use of the modified dairy protein of any preceding claim, or produced by the method of any preceding claim, in preparation of a food or beverage product or ingredient.
Dependencies are inferred from the claim text.