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US 2025/0059241 A1Published application
Compositions and methods for screening insecticidal proteins
AssigneesSyngenta Crop Protection AG, Hokkaido University
InventorsSachiko KASHOJIYA, Shin-ichiro ASANO, Takashi YAMAMOTO
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Abstract
Provided herein are methods for screening for and identifying insecticidal proteins along with recombinant nucleic acids, DNA constructs, vectors, fusion proteins, and spores useful in the provided methods. The provided methods enable effective, high throughput screening of candidate insecticidal proteins, including naturally occurring and engineered synthetic proteins.
Claims34
A recombinant nucleic acid encoding a fusion protein, wherein the recombinant nucleic acid comprises:
- a) a first nucleic acid sequence encoding a spore outer coat polypeptide;
- b) a second nucleic acid sequence encoding a linker;
- and
- c) a third nucleic acid sequence encoding a putative insecticidal polypeptide.
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Dependencies are inferred from the claim text.