Genome-wide classifiers for detection of subacute transplant rejection and other transplant conditions
AssigneesThe Scripps Research Institute, Northwestern University
InventorsSunil M. Kurian, Michael M. Abecassis, John J. Friedewald
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Abstract
This disclosure provides methods of detecting sub-acute rejection and other categories of rejection in kidney transplant recipients using unique sets of gene expression markers.
Claims21
1-102. (canceled)
A method for preparing a cDNA sample from a transplant recipient for distinguishing a non-transplant excellent organ from a transplant excellent organ, comprising:
- (a) extracting RNA from a blood sample of the transplant recipient;
- (b) synthesizing cDNA from the RNA extracted in step (a);
- (c) analyzing the cDNA synthesized in step (b) comprising performing a microarray assay, sequencing assay, or qPCR assay on the cDNA and detecting gene expression levels of FCGR1B, MALAT1, TCF7L2, and LPP;
- (d) applying a trained algorithm of the gene expression levels determined in (c) and distinguishing the non-transplant excellent organ from the transplant excellent organ based on the gene expression levels determined in step (c).
A method for preparing a cDNA sample from a kidney transplant recipient for distinguishing a non-transplant excellent kidney from a transplant excellent kidney, comprising:
- (a) extracting RNA from a blood sample of the transplant recipient;
- (b) synthesizing cDNA from the RNA extracted in step (a);
- (c) analyzing the cDNA synthesized in step (b) comprising performing a microarray assay, sequencing assay, or qPCR assay on the cDNA and detecting gene expression levels of CXXC5, BMP2K, IGKC, GNB1, SSBP3, and ALDH9A1;
- (d) applying a trained algorithm of the gene expression levels determined in (c) and distinguishing the non-transplant excellent kidney from the transplant excellent kidney based on the gene expression levels determined in step (c).
A method for preparing a cDNA sample from a liver transplant recipient for distinguishing a non-transplant excellent liver from a transplant excellent liver, comprising:
- (a) extracting RNA from a blood sample of the transplant recipient;
- (b) synthesizing cDNA from the RNA extracted in step (a);
- (c) analyzing the cDNA synthesized in step (b) comprising performing a microarray assay, sequencing assay, or qPCR assay on the cDNA and detecting gene expression levels of FCGR1B, MALAT1, TCF7L2, and LPP;
- (d) applying a trained algorithm of the gene expression levels determined in (c) and distinguishing the non-transplant excellent liver from the transplant excellent liver based on the gene expression levels determined in step (c).
Dependencies are inferred from the claim text.