Surface coating for spheroid growth
OwnerAman Russom, Harisha Ramachandraiah +1inventors
InventorsAman Russom, Harisha Ramachandraiah, Torbjörn Pettersson
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Abstract
The present invention in a first aspect relates to a method for generating three-dimensional multicellular spheroids from at least one eukaryotic cell, comprising seeding at least one cell onto a scaffold comprising at least one bi-layer comprising a first layer and a second layer (106) with alternating charges, said first layer comprising a polyelectrolyte being biocompatible with said cells and said second layer comprising nanocellulose.
Claims18
1-10. (canceled)
A scaffold for generating three-dimensional multicellular spheroids from at least one eukaryotic cell, the scaffold comprising:
- a first layer and a second layer with alternating charges, said first layer comprising a biocompatible polyelectrolyte and said second layer comprising nanocellulose, wherein the polyelectrolyte is a positively charged polyelectrolyte or a negatively charged polyelectrolyte.
The scaffold according to 11, wherein at least one outermost bi-layer has an outermost layer comprising nanocellulose.
Dependencies are inferred from the claim text.