Methods and compositions for preparing an arrayed library of cells

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A system for selecting and transferring cells, comprising:

• a) an automated apparatus for automatic handling of one or more pick tools and configured for performing automated selection of colonies of cells into one or more locations of a first receptacle, wherein the automated apparatus comprises a solid barrier comprising an opening for an active pick tool to access the colony source and the first receptacle, and wherein the solid barrier is mounted on the automated apparatus and configured to prevent transfer of cells from an inactive pick tool into an undesired location of the first receptacle;
• b) the first receptacle, comprising a high-density media;
• c) an acoustic liquid handler configured to acoustically transfer a portion of the cells from the first receptacle to one or more locations of a second receptacle.

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A method of acoustically transferring cells, comprising:

• a) obtaining a first receptacle comprising one or more cells in a high-density media;
• and
• b) acoustically transferring, using an acoustic liquid handler, at least one of said one or more cells in the high-density media from the first receptacle to one or more locations of a second receptacle.

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A system for acoustically transferring cells, comprising:

• a) one or more cells in a first receptacle comprising a high-density media;
• b) an acoustic liquid handler configured to acoustically transfer at least one of said one or more cells from the first receptacle to one or more locations of a second receptacle.

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A method for reducing contamination during automated colony selection, comprising selecting colonies of cells from a colony source and depositing the colonies in one or more locations of a destination receptacle using an automated apparatus for automatic handling of one or more pick tools, wherein the automated apparatus comprises a solid barrier comprising an opening for an active pick tool to access the colony source and the destination receptacle, and wherein the solid barrier is mounted on the automated apparatus and configured to prevent transfer of cells from an inactive pick tool into an undesired location of the destination receptacle.

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