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AssigneeColorado State University Research Foundation
InventorsStephen Pearce, Caitlynd Krosch, Andrew Katz, Rocio Alarcon Reverte, Karl Ravet, Forrest Wold-McGimsey
Estimating the term…
The disclosure relates to compositions and methods for reducing free asparagine concentration in the grain of wheat plants. The compositions comprise wheat plants, plant parts, plant cells, and seeds comprising a loss-of-function mutation in at least one endogenous ASPARAGINE SYNTHETASE 2 (ASN2) gene. The grain of wheat plants has reduced acrylamide-forming potential. Low acrylamide products produced from the grain are also provided.
A modified wheat plant, or a progeny thereof, comprising a loss-of-function mutation in at least one endogenous ASPARAGINE SYNTHETASE 2 (ASN2) gene selected from ASN-A2, ASN-B2, and ASN-D2, wherein the mutation confers reduced free asparagine concentration in the grain of the wheat plant.
A method of reducing free asparagine concentration in the grain of a wheat plant, the method comprising:
A primer pair having the nucleotide sequences of SEQ ID NOs:
A guide RNA for editing an ASN2 gene comprising the nucleotide sequence of SEQ ID NO:
A nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
Dependencies are inferred from the claim text.