Ethylene receptors and binding domains with modified binding kinetics for ethylene

Abstract

Embodiments of the present disclosure pertain to an ethylene sensor with an ethylene recognition component and a detecting component. The ethylene recognition component includes at least one ethylene binding domain. The detecting component is operational to generate a detectable signal that correlates to the binding of ethylene to the ethylene binding domain. The ethylene binding domain includes: an Asn residue; at least one variant region that reduces the binding affinity of ethylene to the ethylene receptor when compared to the ethylene binding domain without the variant region; at least one mutation that reduces the binding affinity of ethylene to the ethylene binding domain when compared to the ethylene binding domain without the mutation; or combinations thereof. Additional embodiments pertain to methods of sensing an ethylene from an environment by utilizing the ethylene sensors. Further embodiments pertain to modified cells that include the ethylene binding domains.

Dependencies are inferred from the claim text.