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US 2025/0027869 A1Published application
Methods for detecting particles present in a cell composition
AssigneeJuno Therapeutics, Inc.
InventorsBrittney Jo LIVINGSTON, Hai YUE
Life of this patent
Estimating the term…
Abstract
Provided are methods of detecting, assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.
Claims20
A method for enumerating a number of residual beads in a T cell composition, the method comprising:
- (a) incubating a sample comprising at least a portion of an input composition comprising one or more T cells that is likely to or may comprise one or more residual beads under conditions to induce lysis of the one or more T cells to produce an output composition;
- (b) exposing the output composition to a magnet or magnetic field to produce an enriched output composition comprising the one or more beads;
- and
- (c) analyzing the enriched output composition or a portion thereof by fluorescence imaging of autofluorescence and by brightfield imaging to enumerate the number of residual beads in the sample,
- wherein the one or more beads is capable of emitting an autofluorescence and wherein
- the one or more residual beads is magnetic or paramagnetic.
3 more elements
A method for enumerating residual beads in a T cell composition, the method comprising:
- (a) incubating a sample with a solution comprising bleach to induce lysis of one or more T cells to produce an output composition, wherein the sample comprises at least a portion of an input composition comprising the one or more T cells and is likely to comprise or may comprise one or more residual beads, wherein the one or more beads is capable of emitting an autofluorescence;
- (b) exposing the one or more residual beads to a magnet or magnetic field to produce an enriched output composition;
- (c) rinsing or washing the enriched output composition with a wash solution, wherein the rinsing or washing comprises pelleting the one or more beads and removing a volume or reducing a volume of the output composition;
- (d) capturing at least one fluorescent image, and at least one brightfield image of the output composition or a portion thereof;
- and
- (e) analyzing the number of image objects from the one or more images for one or more parameters selected from size, area, fluorescent intensity, brightfield intensity, and/or circularity, thereby enumerating the residual beads,
- wherein the one or more beads is capable of emitting an autofluorescence wherein the one or more residual beads is magnetic or paramagnetic.
4 more elements
Dependencies are inferred from the claim text.